contributed resources for the analysis of the F2 population and support in the form of salaries for authors WLH and DB, but did not have any additional role in the study design, data collection and analysis, decision to publish, or preparation of the manuscript. moschata 'Burpee's Butterbush' F2 population. Duane Bell was employed by Rupp Seeds Inc and received salary support during which time he generated and phenotyped the C. (a company that develops and distributes powdery mildew-resistant squash and pumpkin cultivars) during the time in which the manuscript was edited and in review. 2013-67013-21232 and was later employed by Rupp Seeds Inc. 2010-85117-20551 and the USDA National Institute of Food and Agriculture, Agriculture and Food Research Initiative Competitive Grant No. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.ĭata Availability: All relevant data are within the paper and its Supporting Information files.įunding: Support for William Holdsworth was provided by USDA National Institute of Food and Agriculture, Plant Breeding and Education Grant No. Received: Accepted: NovemPublished: December 9, 2016Ĭopyright: © 2016 Holdsworth et al. PLoS ONE 11(12):Įditor: David Gent, US Department of Agriculture, UNITED STATES The interval was refined to a final candidate interval of 76.4 kb and CAPS markers were developed inside this interval to facilitate marker-assisted selection.Ĭitation: Holdsworth WL, LaPlant KE, Bell DC, Jahn MM, Mazourek M (2016) Cultivar-Based Introgression Mapping Reveals Wild Species-Derived Pm-0, the Major Powdery Mildew Resistance Locus in Squash. pepo cultivars and with an independent F 2 population derived from C. pepo cultivar panel that included the Cornell lines, heirlooms, and 68 additional C. The contribution of this interval to powdery mildew resistance was confirmed by association mapping in a C. A single 516.4 kb wild-derived introgression was present in all of the resistant cultivars and absent in a diverse set of heirlooms that predated the Pm-0 introgression. High marker density was achieved using genotyping-by-sequencing, which yielded over 50,000 de novo SNP markers in each of the three Cucurbita species genotyped. ![]() moschata cultivars bred at Cornell University that, by common possession of Pm-0, form a shared-trait introgression panel. martinezii-derived single nucleotide polymorphism (SNP) alleles in a set of taxonomically and morphologically diverse and resistant C. At present, this gene, Pm-0, is used extensively in breeding, and is found in nearly all powdery mildew-resistant C. Genetic resistance to the disease is not known to occur naturally within Cucurbita pepo and only infrequently in Cucurbita moschata, but has been achieved in both species through the introgression of a major resistance gene from the wild species Cucurbita okeechobeensis subsp. Powdery mildew is a major fungal disease on squash and pumpkin ( Cucurbita spp.) in the US and throughout the world.
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